Sustainable decommissioning of an offshore wind farm

The offshore wind industry has historically focused on setting up new projects, with the decommissioning phase receiving little attention. This can cause future problems as decommissioning needs to be planned at the beginning to prevent complications that may arise, as it implies important operations and high costs. There are numerous features that make decommissioning a challenge, such as the marine environment, the technical limitations of vessels and the lack of specific regulations that determine what should be done, increasing the uncertainty of the process. Additionally, the unique characteristics of the sites involve exclusive optimal solutions for each project. This article analyses the main operation parameters that affect the decommissioning process, identifying the benefits and drawbacks of the influencing variables. A model is designed to compare different transportation strategies, searching for cost reduction. A decommissioning methodology is been proposed based on this analysis, taking into consideration the technical aspects of the process, and minimising environmental impacts. The model forecasts that the predicted duration and costs of this process are not being adequately captured in site decommissioning plans

The aerodynamics of electric arcs in axial flow

This thesis investigates the behaviour of a d.c. electric arc under the influence of a number of different axial gas flow conditions. The work is directed towards obtaining a greater understanding of the electric arcs produced within gas blast circuit breakers. In order to eliminate as many variables as possible, detailed experiments have been carried out in constant pressure, subsonic nitrogen flow provided by a low Mach number shock tube. This facility enables the flow velocity and pressure to be varied independently, thus allowing their effect on arc behaviour to be studied separately. A simplified theoretical model of the arc in this flow field has been developed which gives excellent agreement with the shock tube experiments. The solution of the theoretical model is obtained in non-dimensional terms as a universal characteristic for the constant pressure axial flow arc

Effect of fluid dynamics and device mechanism on biofluid behaviour in microchannel systems: modelling biofluids in a microchannel biochip separator

Biofluid behaviour in microchannel systems is investigated in this paper through the modelling of a microfluidic biochip developed for the separation of blood plasma. Based on particular assumptions, the effects of some mechanical features of the microchannels on behaviour of the biofluid are explored. These include microchannel, constriction, bending channel, bifurcation as well as channel length ratio between the main and side channels. The key characteristics and effects of the microfluidic dynamics are discussed in terms of separation efficiency of the red blood cells with respect to the rest of the medium. The effects include the Fahraeus and Fahraeus-Lindqvist effects, the Zweifach-Fung bifurcation law, the cell-free layer phenomenon. The characteristics of the microfluid dynamics include the properties of the laminar flow as well as particle lateral or spinning trajectories. In this paper the fluid is modelled as a single-phase flow assuming either Newtonian or Non-Newtonian behaviours to investigate the effect of the viscosity on flow and separation efficiency. It is found that, for a flow rate controlled Newtonian flow system, viscosity and outlet pressure have little effect on velocity distribution. When the fluid is assumed to be Non-Newtonian more fluid is separated than observed in the Newtonian case, leading to reduction of the flow rate ratio between the main and side channels as well as the system pressure as a whole

The conceptual design of 3D miniaturised / integrated products as examined through the development of a novel red blood cell / plasma separation device

This thesis was submitted for the award of Doctor of Philosophy and was awarded by Brunel University LondonThe aim of this research is to examine the conceptual design issues concerned with integrating product capabilities that can only be generated at the micro- scale (through feature sizes generally of the order of 100nm to 100μm) directly into 3-dimensional products at the macro-scale. Such macro-scale products could accordingly contain internal devices that are too small to be seen or touched by unaided human designers, which begs the question as to how to enable designers to work with objects which are beyond direct human experience, and how can the necessary collective discussion take place within teams of designers, and between these teams and those responsible for product manufacture? This thesis examines and tests a concept that theoretical 2-dimensional diagrams of function may be transformed into 3-dimensional working structures using procedures allied to those used by graphic designers to create solid objects from 2-dimensional prototype geometries through, for example, extrusion or rotation. Applying such procedures to theoretical diagrams in order to transform them into scalable 3-dimensional devices is not yet in general use at the macro-scale, but with increasing recognition of the unique capabilities of the micro- scale the idea may grow in appeal to alleviate the difficulties of conceiving of functional structures that, when built, will be too small to experience directly. Furthermore this design method, through its basis upon a common currency of functional diagrams, may overcome many of the problems of describing and discussing the design and manufacture of normally intangible objects in 3 dimensions. Finally, it is shown through the example of a novel Red Blood Cell / Plasma Separation Device that the geometric transformation process can lead to the design of functional structures which would not readily be arrived at intuitively, and that may be effectively and efficiently integrated into host products

Response to Scottish Government's 'Draft Offshore Wind Policy Statement: Consultation'

This is a combined response to the Scottish Government's consultation on its Draft Offshore Wind Policy Statement

The K186E amino acid substitution in the canine influenza virus H3N8 NS1 protein restores its ability to inhibit host gene expression

Canine influenza viruses (CIVs) are the causative agents of canine influenza, a contagious respiratory disease in dogs, and include the equine-origin H3N8 and the avian-origin H3N2. Influenza A virus (IAV) non-structural protein 1 (NS1) is a virulence factor essential for counteracting the innate immune response. Here, we evaluated the ability of H3N8 CIV NS1 to inhibit host innate immune responses. We found that H3N8 CIV NS1 was able to efficiently counteract interferon (IFN) responses but was unable to block general gene expression in human or canine cells. Such ability was restored by a single amino acid substitution in position 186 (K186E) that resulted in NS1 binding to the 30-kDa subunit of the cleavage and polyadenylation specificity factor (CPSF30), a cellular protein involved in pre-mRNA processing. We also examined the frequency distribution of K186 and E186 among H3N8 CIVs and equine influenza viruses (EIVs), the ancestors of H3N8 CIV, and experimentally determined the impact of amino acid 186 in the ability of different CIV and EIV NS1s to inhibit general gene expression. In all cases, the presence of E186 was responsible for the control of host gene expression. Contrastingly, the NS1 protein of H3N2 CIV harbors E186 and blocks general gene expression in canine cells. Altogether, our results confirm previous studies on the strain-dependent ability of NS1 to block general gene expression. Moreover, the observed polymorphism on amino acid 186 between H3N8 and H3N2 CIVs might be the result of adaptive changes acquired during long-term circulation of avian-origin IAVs in mammals. IMPORTANCE: Canine influenza is a respiratory disease of dogs caused by two CIV subtypes, the H3N8 and H3N2 viruses of equine and avian origin, respectively. Influenza NS1 is the main viral factor responsible for the control of host innate immune responses and changes in NS1 can play an important role in host adaptation. Here we assessed the ability of H3N8 CIV NS1 to inhibit host innate immune responses and gene expression. The H3N8 CIV NS1 did not block host gene expression but this activity was restored by a single amino acid substitution (K186E), which was responsible for NS1 binding to the host factor CPSF30. In contrast, the H3N2 CIV NS1, that contains E186, blocks general gene expression. Our results suggest that the ability to block host gene expression is not required for influenza replication in mammals but might be important in the long-term adaptation of avian-origin influenza viruses to mammals

A temperature sensitive live-attenuated canine influenza virus H3N8 vaccine

Canine influenza is a respiratory disease of dogs caused by canine influenza virus (CIV). CIV subtypes responsible for influenza in dogs include H3N8, which originated from the transfer of H3N8 equine influenza virus to dogs; and the H3N2 CIV, which is an avian-origin virus that adapted to infect dogs. Influenza infections are most effectively prevented through vaccination to reduce transmission and future infection. Currently, only inactivated influenza vaccines (IIVs) are available for the prevention of CIV in dogs. However, the efficacy of IIVs is suboptimal, and novel approaches are necessary for the prevention of disease caused by this canine respiratory pathogen. Using reverse genetics techniques, we have developed a live-attenuated CIV vaccine (LACIV) for the prevention of H3N8 CIV. The H3N8 LACIV replicates efficiently in canine cells at 33°C but is impaired at temperatures of 37 to 39°C and was attenuated compared to wild-type H3N8 CIV in vivo and ex vivo. The LACIV was able to induce protection against H3N8 CIV challenge with a single intranasal inoculation in mice. Immunogenicity and protection efficacy were better than that observed with a commercial CIV H3N8 IIV but provided limited cross-reactive immunity and heterologous protection against H3N2 CIV. These results demonstrate the feasibility of implementing a LAIV approach for the prevention and control of H3N8 CIV in dogs and suggest the need for a new LAIV for the control of H3N2 CIV. Importance: Two influenza A virus subtypes has been reported in dogs in the last 16 years: the canine influenza viruses (CIV) H3N8 and H3N2 of equine and avian origins, respectively. To date, only inactivated influenza vaccines (IIVs) are available to prevent CIV infections. Here, we report the generation of a recombinant, temperature-sensitive H3N8 CIV as a live-attenuated influenza vaccine (LAIV), which was attenuated in mice and dog tracheal, explants compared to CIV H3N8 wild type. A single dose of H3N8 LACIV showed immunogenicity and protection against a homologous challenge that was better than that conferred with an H3N8 IIV, demonstrating the feasibility of implementing a LAIV approach for the improved control of H3N8 CIV infections in dogs

An Early CD4+ T Cell–dependent Immunoglobulin A Response to Influenza Infection in the Absence of Key Cognate T–B Interactions

Contact-mediated interactions between CD4+ T cells and B cells are considered crucial for T cell–dependent B cell responses. To investigate the ability of activated CD4+ T cells to drive in vivo B cell responses in the absence of key cognate T–B interactions, we constructed radiation bone marrow chimeras in which CD4+ T cells would be activated by wild-type (WT) dendritic cells, but would interact with B cells that lacked expression of either major histocompatibility complex class II (MHC II) or CD40. B cell responses were assessed after influenza virus infection of the respiratory tract, which elicits a vigorous, CD4+ T cell–dependent antibody response in WT mice. The influenza-specific antibody response was strongly reduced in MHC II knockout and CD40 knockout mice. MHC II–deficient and CD40-deficient B cells in the chimera environment also produced little virus-specific immunoglobulin (Ig)M and IgG, but generated a strong virus-specific IgA response with virus-neutralizing activity. The IgA response was entirely influenza specific, in contrast to the IgG2a response, which had a substantial nonvirus-specific component. Our study demonstrates a CD4+ T cell–dependent, antiviral IgA response that is generated in the absence of B cell signaling via MHC II or CD40, and is restricted exclusively to virus-specific B cells

Challenges of decommissioning offshore wind farms : overview of the European experience

In the coming years, an important number of offshore wind turbines will reach the end of their initially planned service life. In a wind turbine end-of-life scenario, owners can decide between extending the life of the asset, repowering the site or decommissioning. This decision-making process is affected by important sources of uncertainty, especially in offshore environments. The limited experience makes the decommissioning procedure challenging, as it is still largely unexplored. This work assesses the current state of knowledge about the challenges surrounding the decommissioning process of an offshore wind farm. The four main challenges encountered are identified and analysed in detail, namely the regulatory framework, the overall planning of the process, the logistics and vessels' availability, and the environmental impacts confronted. Ultimately, this paper aims at stimulating the dialogue among stakeholders and raising the awareness of adequately regulating and preparing the upcoming decommissioning of offshore wind farms in Europe